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Structured Review

Bethyl anti wdr5
Anti Wdr5, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+wdr5/WDR5+Antibody/pm41634442-437-37-40
Average 94 stars, based on 101 article reviews
anti wdr5 - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Staining:

Article Title: MLL/WDR5 complex recruits centriolar satellite protein Cep72 to regulate microtubule nucleation and spindle formation
Article Snippet: .. Cells were blocked using 1% bovine serum albumin for 1 hour at room temperature and stained with primary antibodies [anti-MLL1: A300-374A and A300-086A, Bethyl Labs; anti-MLL C D1 ( ); anti-WDR5: A310-880A, Bethyl Labs; anti-PCNT: ab4448 and ab28144, Abcam; anti-NEDD1: ab57336, Abcam; anti–γ-tubulin: GTU-88, T6557, Sigma-Aldrich; anti–α-tubulin: T9026, Sigma-Aldrich; anti-Cep72: A301-297A, Bethyl Labs; anti-Cep72: 19928-1-AP, Proteintech; anti-GCP2: PA5-21433, Thermo Fisher Scientific; anti-AKAP9: 611518, BD Laboratories; anti-CDK5RAP2: ab70213; and anti-FLAG: F7425, Sigma-Aldrich] and conjugated secondary antibodies (Alexa Fluor 488: A11029 and A11034; Alexa Fluor 568: A11031 and A11036; Alexa Fluor 594: A11032 and A11037; or Alexa Fluor 633, A21070 and A21050, Invitrogen). ..

Western Blot:

Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
Article Snippet: .. For western blotting, the following primary antibodies were used at 1:1,000 dilution unless otherwise specified: anti-OGT (Proteintech, 11576-2-AP), anti-HA (C29F4), anti-Nup62 (BD Biosciences, 610498), anti-HCFC1 (Bethyl Laboratories, A301-399A-M), anti-SET1A (61702S), anti-RBBP5 (13171S), anti-BAP1 (rabbit, Bethyl Laboratories, A302-243A-M), anti-WDR5 (13105S), anti-FoxK1 (Bethyl Laboratories, A301-728A-M), anti-FoxK2 (12008S), anti-liprin-α1 (Proteintech, 14175-1-AP), anti-BAP1 (mouse, Santa Cruz Biotechnology, sc-28383), anti-O-GlcNAc (RL-2, Abcam, ab2739, 1:500) or anti-α-tubulin (MilliporeSigma, T9026, 1:3,000 or Thermo Fisher Scientific, 62204, 1:1,000). ..

other:

Article Title: MLL/WDR5 complex recruits KIF2C to midbody to ensure MT depolymerization and furrow compaction during cytokinesis
Article Snippet: Various antibodies used in our experiments are as follows: Anti-WDR5 (Bethyl, A302-430A), Anti-MLLc (Bethyl, A300-374A), Anti-MLLc D1 , anti-GFP (Invitrogen, A11122), anti-RbBP5 (Bethyl, A300-109A), anti-KIF2C (protein-tech, 28372-1-AP), anti-α-tubulin (Sigma-Aldrich, T1568), anti-RACGAP1 (Cloud-Clone corp., PAE293Hu01), anti-Annilin (Cloud-clone corp., PAJ640Hu01), Alexa fluor 488 (Invitrogen, A11029, A11034), Alexa fluor 594 (Invitrogen, A11032, A11037, A21029) and Horse radish peroxidase (HRP) conjugated secondary antibodies (Bio-Rad, 170-6516, Bio-Rad, 170-6515).

Immunoprecipitation:

Article Title: MLL/WDR5 complex recruits centriolar satellite protein Cep72 to regulate microtubule nucleation and spindle formation
Article Snippet: .. Immunoprecipitation of endogenous proteins was performed using anti-MLL C (05-765, EMD Millipore) or anti-WDR5 (A302-430A, Bethyl Labs) or anti-Cep72 (A301-297A, Bethyl Labs) antibody overnight at 4°C incubated with precleared HeLa cell lysate prepared in lysis buffer [100 mM NaCl, 20 mM tris (pH 7.4), 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 1 mM DTT, aprotenin (1 μg/ml), and pepstatin (1 μg/ml)], followed by incubation with Protein A Sepharose beads (Cytiva) for 2 hours. ..

Incubation:

Article Title: MLL/WDR5 complex recruits centriolar satellite protein Cep72 to regulate microtubule nucleation and spindle formation
Article Snippet: .. Immunoprecipitation of endogenous proteins was performed using anti-MLL C (05-765, EMD Millipore) or anti-WDR5 (A302-430A, Bethyl Labs) or anti-Cep72 (A301-297A, Bethyl Labs) antibody overnight at 4°C incubated with precleared HeLa cell lysate prepared in lysis buffer [100 mM NaCl, 20 mM tris (pH 7.4), 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 1 mM DTT, aprotenin (1 μg/ml), and pepstatin (1 μg/ml)], followed by incubation with Protein A Sepharose beads (Cytiva) for 2 hours. ..

Lysis:

Article Title: MLL/WDR5 complex recruits centriolar satellite protein Cep72 to regulate microtubule nucleation and spindle formation
Article Snippet: .. Immunoprecipitation of endogenous proteins was performed using anti-MLL C (05-765, EMD Millipore) or anti-WDR5 (A302-430A, Bethyl Labs) or anti-Cep72 (A301-297A, Bethyl Labs) antibody overnight at 4°C incubated with precleared HeLa cell lysate prepared in lysis buffer [100 mM NaCl, 20 mM tris (pH 7.4), 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 1 mM DTT, aprotenin (1 μg/ml), and pepstatin (1 μg/ml)], followed by incubation with Protein A Sepharose beads (Cytiva) for 2 hours. ..



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